Leaf Chromatography: Hidden Colors in Leaves
Discover science with Discovery Lab

Why do leaves change color in fall? Even green leaves contain many pigments – you just can’t always see them. With a simple chromatography experiment, you can uncover the hidden colors.
What you will need:
- Fresh leaves
- Small jars or cups
- Rubbing alcohol
- Coffee filters
- Scissors
- Warm water bath (bowl of hot water)
Step-by-step
- Tear leaves into small pieces and place them in jars.
- Add rubbing alcohol and mash leaves gently.
- Place jars in a warm-water bath for 30 minutes.
- Insert strips of coffee filter so the tips touch the liquid.
- Watch as colors climb the paper, then let strips dry.
The science behind it!
Leaves contain chlorophyll (green), carotenoids (yellow/orange), and anthocyanins (red/purple). In summer, green chlorophyll hides the others. Chromatography separates these pigments, revealing the “secret rainbow” inside every leaf.